ROS-Nitric Oxide Pathway Reagents and Kits
Enzo Life Sciences VASP monoclonal antibody (IE273) (50µg)
Clone: IE273. Isotype: IgG1. Immunogen: Purified human VASP (vasodilator stimulated phosphoprotein). Species reactivity: Human Rabbit, Porcine, Bovine. Specificity: Recognizes both the 46kDa (Ser157 dephospho-) and 50kDa (Ser157 phospho-) form of VASP. Applications: ICC, IP, WB. Formulation: Liquid. In PBS containing 2mg/ml BSA and 0.02% sodium azide.
Enzo Life Sciences SNAP (100mg). CAS: 79032-48-7
Stable analog of endogenous S-nitroso compounds. Nitric oxide (NO) donor and a source of NO in vivo which spontaneously releases NO under physiological conditions. The half-life time is 4.6 hours in aqueous media. Potent vasodilator in vitro and in vivo that does not induce pharmacological tolerance. Relaxes isolated bovine coronary artery rings with an EC50=0.1µM. It also inhibits vascular smooth muscle cell mitogenesis and proliferation, inhibits leukocyte adherence to endothelium, and inhibits cysteine proteases. The unique NO releasing properties of SNAP make it an ideal agent for studying the pharmacological and physiological actions of NO. Alternative name: S-Nitroso-N-acetyl-D,L-penicillamine. Purity: ≥98% (HPLC). Solubility: Soluble in DMSO (25mg/ml) 100% ethanol (25mg/ml) or methanol; slightly soluble in water (2mg/ml). Long Term Storage: -20°C.
Abcam Alkaline Phosphatase Assay Kit (Fluorometric)
Alkaline Phosphatase Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences TranscreenerR ADP2 FI Assa
The Transcreener(R) HTS Assay platform overcomes the need for time-consuming one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener(R) HTS Assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families. The Transcreener(R) ADP2 fluorescent intensity (FI) assay extends the Transcreener(R) platform for ADP detection by utilizing a simple fluorescent intensity output which can be used on both fluorescence readers typically found in academic and therapeutic research labs as well as more complex multimode plate readers more commonly used in core facilities and HTS labs. The Transcreener(R) ADP2 FI Assay is a red competitive fluorescence intensity (FI) assay based on the detection of ADP and therefore is compatible with any enzyme class
Abcam 6-FAM (6-Carbo x yfluorescein), Amine-reactive fluorescent label, 50MG
MW 376.3 Da, Purity >97%. Amine-reactive fluorescent label. 5-FAM (ab145293) isomer. 6-CFDA (ab145298) and 6-CFDA N-succinimidyl ester (ab145296) hydrolytic product. Polar tracer. Covalently labels peptides and proteins. Deprotonates below pH 7 resulting in reduced fluorescence. Compatible with most fluorescence detection equipment.
The product is subject to the following: Abcam Restricted Use Statement
Abcam 6-FAM (6-Carbo x yfluorescein), Amine-reactive fluorescent label, 1G
MW 376.3 Da, Purity >97%. Amine-reactive fluorescent label. 5-FAM (ab145293) isomer. 6-CFDA (ab145298) and 6-CFDA N-succinimidyl ester (ab145296) hydrolytic product. Polar tracer. Covalently labels peptides and proteins. Deprotonates below pH 7 resulting in reduced fluorescence. Compatible with most fluorescence detection equipment.
The product is subject to the following: Abcam Restricted Use Statement
Abcam 7-Metho x ycoumarin-3-carbo x ylic acid, Amine-reactive fluorescent probe, 500MG
MW 220.18 g/mol, Purity >98%. Amine-reactive fluorescent probe. Used for coupling to amines, hydrazines or hydroxylamines on biomolecules of interest.
The product is subject to the following: Abcam Restricted Use Statement
Abcam DAF-2, Fluorescent nitric o x ide probe, 1MG
MW 362.34 g/mol, Purity >95%. Highly sensitive, specific fluorescent detector for nitric oxide (NO). Essentially nonfluorescent until it reacts with NO to yield the highly fluorescent triazolofluorescein (DAF-2T) (ab145473).
The product is subject to the following: Abcam Restricted Use Statement
Abcam DAF-2, Fluorescent nitric o x ide probe, 5MG
MW 362.34 g/mol, Purity >95%. Highly sensitive, specific fluorescent detector for nitric oxide (NO). Essentially nonfluorescent until it reacts with NO to yield the highly fluorescent triazolofluorescein (DAF-2T) (ab145473).
The product is subject to the following: Abcam Restricted Use Statement
Abcam DMNQ, non-alkyiating redo x cycling quinone, 5MG
MW 218.2 Da, Purity >99%. Cell-permeable, non-alkylating redox cycling quinone. Generates superoxide production and induces cell proliferation, apoptosis and necrosis in vitro. Redox cycling agent. Active in vivo and in vitro.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Histone H4 Total Acetylation Assay Kit (Fluorometric).
Histone H4 Total Acetylation Assay Kit (Fluorometric).
The product is subject to the following: Abcam Restricted Use Statement